34-8800

antibody from Invitrogen Antibodies

Targeting: KIT C-Kit, CD117, PBT, SCFR

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

34-8800

Provider

Invitrogen Antibodies

Product name

c-Kit Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

0.25 mg/mL

Storage

-20°C

References

Submitted references

Protective effect of thymoquinone against lung intoxication induced by malathion inhalation.

Abdo W, Elmadawy MA, Abdelhiee EY, Abdel-Kareem MA, Farag A, Aboubakr M, Ghazy E, Fadl SE
Scientific reports 2021 Jan 28;11(1):2498

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Telocytes in the mouse testicular interstitium: implications of G-protein-coupled estrogen receptor (GPER) and estrogen-related receptor (ERR) in the regulation of mouse testicular interstitial cells.

Pawlicki P, Hejmej A, Milon A, Lustofin K, Płachno BJ, Tworzydlo W, Gorowska-Wojtowicz E, Pawlicka B, Kotula-Balak M, Bilinska B
Protoplasma 2019 Mar;256(2):393-408

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Hypoxia-regulated delta-like 1 homologue enhances cancer cell stemness and tumorigenicity.

Kim Y, Lin Q, Zelterman D, Yun Z
Cancer research 2009 Dec 15;69(24):9271-80

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A cellular study of human testis development.

Ostrer H, Huang HY, Masch RJ, Shapiro E
Sexual development : genetics, molecular biology, evolution, endocrinology, embryology, and pathology of sex determination and differentiation 2007;1(5):286-92

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of c-Kit/CD117 was performed using 70% confluent log phase SH-SY5Y cells treated with 10 uM retinoic acid for 72 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with c-Kit/CD117 Rabbit Polyclonal Antibody (Product # 34-8800) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjµgate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membranous localization. Panel e is untreated cell with less signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of c-Kit / CD117 was done on K-562 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with c-Kit / CD117 Rabbit Polyclonal Antibody (348800, red histogram) or with rabbit isotype control (pink histogram) at 3-5 µg/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control..

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 Photomicrograph of the pulmonary sections of the different groups immunostained with C-KIT antibody. (A) The lung of G1 and (B) lung of G2 showing scarcely expression of C-KIT antibody within the pulmonary tissues. (C) Lung of G3 showing numerous C-KIT positive mast cells (arrows). (D) Lung of G4 showing decrease of C-KIT positive mast cells. (E) Lung of G5 showing marked decrease C-KIT positive mast cells, bar = 50 um. (F) Quantitative score of the number of C-KIT positive mast cells. Y= indicates significance in comparing with G1 (P < 0.001), * indicates significance in comparison with G3 (P < 0.001) and # indicates significance in comparison with G4 (P < 0.05).