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- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
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- Product number
- 44-492 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Phospho-c-Kit (Tyr703) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20°C
Submitted references Autophagy is involved in endogenous and NVP-AUY922-induced KIT degradation in gastrointestinal stromal tumors.
The identification and characterisation of novel KIT transcripts in aggressive mast cell malignancies and normal CD34+ cells.
Lyn contributes to regulation of multiple Kit-dependent signaling pathways in murine bone marrow mast cells.
Hsueh YS, Yen CC, Shih NY, Chiang NJ, Li CF, Chen LT
Autophagy 2013 Feb 1;9(2):220-33
Autophagy 2013 Feb 1;9(2):220-33
The identification and characterisation of novel KIT transcripts in aggressive mast cell malignancies and normal CD34+ cells.
Ozer O, Zhao YD, Ostler KR, Akin C, Anastasi J, Vardiman JW, Godley LA
Leukemia & lymphoma 2008 Aug;49(8):1567-77
Leukemia & lymphoma 2008 Aug;49(8):1567-77
Lyn contributes to regulation of multiple Kit-dependent signaling pathways in murine bone marrow mast cells.
Shivakrupa R, Linnekin D
Cellular signalling 2005 Jan;17(1):103-9
Cellular signalling 2005 Jan;17(1):103-9
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot using CD117 (c-Kit) (pY703) Polyclonal Antibody, Rabbit
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Peptide Competition: Extracts prepared from M07e cells left untreated (1) or treated (2-5) with SCF were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with 0.50 µg/mL c-Kit (pY703) antibody for two hours at room temperature in a 1% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phospho-tyrosine containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F (ab’)2 anti-rabbit IgG alkaline phosphatase (Product # ALI4405) and signals were detected using the Tropix WesternStar™ method. The data show that only the peptide corresponding to c-Kit (pY703) blocks the antibody signal, thereby demonstrating the specificity of the antibody.
