12-1179-42

antibody from Invitrogen Antibodies
Targeting: KIT C-Kit, CD117, PBT, SCFR
Provider product page for 12-1179-42

Antibody data

Product number
12-1179-42 - Provider product page
Provider
Invitrogen Antibodies
Product name
CD117 (c-Kit) Monoclonal Antibody (YB5.B8), PE, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The YB5.B8 monoclonal antibody reacts with human CD117, also known as c-Kit, Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by hematopoietic progenitor cell subsets and mast cells. The interaction of c-Kit and Steel factor promotes proliferation and differentiation of hematopoietic progenitor cells and mast cell differentiation. CD117 is also expressed by melanocytes and plays a role in signaling and activation of these cells. When interested in staining HSCs from peripheral blood, we recommend the use of clone 104D2. Please refer to Product # 17-1178. Applications Reported: YB5.B8 has been reported for use in flow cytometric analysis. Applications Tested: The YB5.B8 antibody has been tested by flow cytometric analysis of the TF-1 cell line, a human erythroleukemia cell line. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Conjugate
Yellow dye
Isotype
IgG
Antibody clone number
YB5.B8
Vial size
100 Tests
Concentration
5 µL/Test
Storage
4° C, store in dark, DO NOT FREEZE!
KIT protein structure - 12-1179-42 shown in red.