14-1179-80

antibody from Invitrogen Antibodies
Targeting: KIT C-Kit, CD117, PBT, SCFR
Provider product page for 14-1179-80
Immunoprecipitation
Immunohistochemistry
Flow cytometry

Antibody data

Product number
14-1179-80 - Provider product page
Provider
Invitrogen Antibodies
Product name
Anti-CD117 (c-Kit) Monoclonal Antibody (YB5.B8), eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The YB5.B8 monoclonal antibody reacts with human CD117, also known as c-Kit , Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by hematopoietic progenitor cell subsets and mast cells. The interaction of c-Kit and Steel factor promotes proliferation and differentiation of hematopoietic progenitor cells and mast cell differentiation. CD117 is also expressed by melanocytes and plays a role in signaling and activation of these cells. Applications Reported: YB5.B8 has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections. Applications Tested: The YB5.B8 antibody has been tested by flow cytometric analysis of the TF-1 cell line, a human erythroleukemia cell line. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
YB5.B8
Vial size
25 µg
Concentration
0.5 mg/mL
Storage
4° C
KIT protein structure - 14-1179-80 shown in red.