Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations [0]
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- Product number
- 14-1179-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD117 (c-Kit) Monoclonal Antibody (YB5.B8), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The YB5.B8 monoclonal antibody reacts with human CD117, also known as c-Kit , Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by hematopoietic progenitor cell subsets and mast cells. The interaction of c-Kit and Steel factor promotes proliferation and differentiation of hematopoietic progenitor cells and mast cell differentiation. CD117 is also expressed by melanocytes and plays a role in signaling and activation of these cells. Applications Reported: YB5.B8 has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections. Applications Tested: The YB5.B8 antibody has been tested by flow cytometric analysis of the TF-1 cell line, a human erythroleukemia cell line. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- YB5.B8
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Immunophenotypic comparison of heterogenous non-sorted versus sorted mononuclear cells from human umbilical cord blood: a novel cell enrichment approach.
Human primordial germ cell commitment in vitro associates with a unique PRDM14 expression profile.
Transcriptional profile of tuberculosis antigen-specific T cells reveals novel multifunctional features.
Neoangiogenesis contributes to the development of hemophilic synovitis.
Epitope mapping and functional studies with three monoclonal antibodies to the c-kit receptor tyrosine kinase, YB5.B8, 17F11, and SR-1.
Assessment of the anti-c-kit monoclonal antibody YB5.B8 in affinity magnetic enrichment of human lung mast cells.
Monoclonal antibody YB5.B8 identifies the human c-kit protein product.
Indumathi S, Harikrishnan R, Rajkumar JS, Dhanasekaran M
Cytotechnology 2015 Jan;67(1):107-14
Cytotechnology 2015 Jan;67(1):107-14
Human primordial germ cell commitment in vitro associates with a unique PRDM14 expression profile.
Sugawa F, Araúzo-Bravo MJ, Yoon J, Kim KP, Aramaki S, Wu G, Stehling M, Psathaki OE, Hübner K, Schöler HR
The EMBO journal 2015 Apr 15;34(8):1009-24
The EMBO journal 2015 Apr 15;34(8):1009-24
Transcriptional profile of tuberculosis antigen-specific T cells reveals novel multifunctional features.
Arlehamn CL, Seumois G, Gerasimova A, Huang C, Fu Z, Yue X, Sette A, Vijayanand P, Peters B
Journal of immunology (Baltimore, Md. : 1950) 2014 Sep 15;193(6):2931-40
Journal of immunology (Baltimore, Md. : 1950) 2014 Sep 15;193(6):2931-40
Neoangiogenesis contributes to the development of hemophilic synovitis.
Acharya SS, Kaplan RN, Macdonald D, Fabiyi OT, DiMichele D, Lyden D
Blood 2011 Feb 24;117(8):2484-93
Blood 2011 Feb 24;117(8):2484-93
Epitope mapping and functional studies with three monoclonal antibodies to the c-kit receptor tyrosine kinase, YB5.B8, 17F11, and SR-1.
Ashman LK, Bühring HJ, Aylett GW, Broudy VC, Müller C
Journal of cellular physiology 1994 Mar;158(3):545-54
Journal of cellular physiology 1994 Mar;158(3):545-54
Assessment of the anti-c-kit monoclonal antibody YB5.B8 in affinity magnetic enrichment of human lung mast cells.
Okayama Y, Hunt TC, Kassel O, Ashman LK, Church MK
Journal of immunological methods 1994 Mar 10;169(2):153-61
Journal of immunological methods 1994 Mar 10;169(2):153-61
Monoclonal antibody YB5.B8 identifies the human c-kit protein product.
Lerner NB, Nocka KH, Cole SR, Qiu FH, Strife A, Ashman LK, Besmer P
Blood 1991 May 1;77(9):1876-83
Blood 1991 May 1;77(9):1876-83
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