Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 17-1179-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD117 (c-Kit) Monoclonal Antibody (YB5.B8), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The YB5.B8 monoclonal antibody reacts with human CD117, also known as c-Kit , Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by hematopoietic progenitor cell subsets and mast cells. The interaction of c-Kit and Steel factor promotes proliferation and differentiation of hematopoietic progenitor cells and mast cell differentiation. CD117 is also expressed by melanocytes and plays a role in signaling and activation of these cells. When interested in staining HSCs from peripheral blood, we recommend the use of clone 104D2. Please refer to cat. 17-1178. Applications Reported: This YB5.B8 antibody has been reported for use in flow cytometric analysis. Applications Tested: This YB5.B8 antibody has been pre-titrated and tested by flow cytometric analysis of the TF-1 cell line, a human erythroleukemia cell line. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- YB5.B8
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Immunophenotypic comparison of heterogenous non-sorted versus sorted mononuclear cells from human umbilical cord blood: a novel cell enrichment approach.
Human primordial germ cell commitment in vitro associates with a unique PRDM14 expression profile.
Lineage depletion of stromal vascular fractions isolated from human adipose tissue: a novel approach towards cell enrichment technology.
Oxidative stress leads to increased mutation frequency in a murine model of myelodysplastic syndrome.
CD45/CD11b positive subsets of adult lung anchorage-independent cells harness epithelial stem cells in culture.
Indumathi S, Harikrishnan R, Rajkumar JS, Dhanasekaran M
Cytotechnology 2015 Jan;67(1):107-14
Cytotechnology 2015 Jan;67(1):107-14
Human primordial germ cell commitment in vitro associates with a unique PRDM14 expression profile.
Sugawa F, Araúzo-Bravo MJ, Yoon J, Kim KP, Aramaki S, Wu G, Stehling M, Psathaki OE, Hübner K, Schöler HR
The EMBO journal 2015 Apr 15;34(8):1009-24
The EMBO journal 2015 Apr 15;34(8):1009-24
Lineage depletion of stromal vascular fractions isolated from human adipose tissue: a novel approach towards cell enrichment technology.
Indumathi S, Mishra R, Harikrishnan R, Rajkumar JS, Kantawala N, Dhanasekaran M
Cytotechnology 2014 Mar;66(2):219-28
Cytotechnology 2014 Mar;66(2):219-28
Oxidative stress leads to increased mutation frequency in a murine model of myelodysplastic syndrome.
Chung YJ, Robert C, Gough SM, Rassool FV, Aplan PD
Leukemia research 2014 Jan;38(1):95-102
Leukemia research 2014 Jan;38(1):95-102
CD45/CD11b positive subsets of adult lung anchorage-independent cells harness epithelial stem cells in culture.
Peter Y, Sen N, Levantini E, Keller S, Ingenito EP, Ciner A, Sackstein R, Shapiro SD
Journal of tissue engineering and regenerative medicine 2013 Jul;7(7):572-83
Journal of tissue engineering and regenerative medicine 2013 Jul;7(7):572-83
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of the TF-1 cell line with Mouse IgG1 kappa Isotype Control APC (Product # 17-4714-81) (open histogram) or Anti-Human CD117 (c-Kit) APC (filled histogram). Total viable cells were used for analysis.