Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 63-1178-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD117 (c-Kit) Monoclonal Antibody (104D2), Super Bright 600, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- The 104D2 monoclonal antibody reacts with human CD117, also known as c-Kit, Steel factor receptor, and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by hematopoietic progenitor cell subsets and mast cells. The interaction of c-Kit and Steel factor promotes proliferation and differentiation of hematopoietic progenitor cells and mast cell differentiation. CD117 is also expressed by melanocytes and plays a role in signaling and activation of these cells. This 104D2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 2-8°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorochrome performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 104D2
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references PPAR-α and glucocorticoid receptor synergize to promote erythroid progenitor self-renewal.
Lee HY, Gao X, Barrasa MI, Li H, Elmes RR, Peters LL, Lodish HF
Nature 2015 Jun 25;522(7557):474-7
Nature 2015 Jun 25;522(7557):474-7
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Staining of normal human peripheral blood cells with the Human Hematopoietic Lineage eFluor® 450 Cocktail (Product # 22-7775-72), Anti-Human CD34 FITC (Product # 11-0349-42) and Mouse IgG1 K Isotype Control Super Bright 600 (Product # 63-4714-82) (left) or Anti-Human CD117 (c-Kit) Super Bright 600 (right). Lineage negative/low cells were used for analysis.