Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Other assay [2]
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- Product number
- 44-498G - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-c-Kit (Tyr823) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Chicken/Avian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20°C
Submitted references Discovery of a highly selective KIT kinase primary V559D mutant inhibitor for gastrointestinal stromal tumors (GISTs).
CADM1 controls actin cytoskeleton assembly and regulates extracellular matrix adhesion in human mast cells.
Lyn contributes to regulation of multiple Kit-dependent signaling pathways in murine bone marrow mast cells.
Yu K, Liu X, Jiang Z, Hu C, Zou F, Chen C, Ge J, Wu J, Liu X, Wang A, Wang W, Wang W, Qi Z, Wang B, Wang L, Yan H, Wang J, Ren T, Tang J, Liu Q, Liu J
Oncotarget 2017 Dec 19;8(67):111110-111118
Oncotarget 2017 Dec 19;8(67):111110-111118
CADM1 controls actin cytoskeleton assembly and regulates extracellular matrix adhesion in human mast cells.
Moiseeva EP, Straatman KR, Leyland ML, Bradding P
PloS one 2014;9(1):e85980
PloS one 2014;9(1):e85980
Lyn contributes to regulation of multiple Kit-dependent signaling pathways in murine bone marrow mast cells.
Shivakrupa R, Linnekin D
Cellular signalling 2005 Jan;17(1):103-9
Cellular signalling 2005 Jan;17(1):103-9
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Extracts of M07e cells unstimulated (1) or stimulated with 250 ng/mL SCF (2-5) incubated with c-Kit (pY823) antibody, after: no peptide (1, 2), non-phosphorylated peptide (3), generic phosphotyrosine-containing peptide (4), phosphopeptide immunogen (5).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Extracts of M07e cells unstimulated (1) or stimulated with 250 ng/mL SCF (2-5) incubated with c-Kit (pY823) antibody, after: no peptide (1, 2), non-phosphorylated peptide (3), generic phosphotyrosine-containing peptide (4), phosphopeptide immunogen (5).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Phospho-c-Kit (Tyr823) Polyclonal Antibody was performed using 70% confluent log phase Jurkat cells treated with 250 ng/mL SCF for 5 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-c-Kit (Tyr823) Rabbit Polyclonal Antibody (Product # 44-498G) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membranous localization. Panel e shows the untreated control cells with no signal. Panel f shows the no primary antibody control. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Modulated CADM1 levels in HMC-1 cells influenced surface Kit expression, an assembly of filamentous actin and tyrosine phosphorylation. A. HMC-1 cells were transduced with SP4, SP1, control shRNA LucSh, CADM1 shRNA (Sh5 or Shm) viral particles. Then examined for expression of surface CADM1 (total n = 30 groups from 7 transductions), surface Kit (n = 27 from 7 transductions) and amounts of F-actin (n = 23 from 4 transductions) by FACS. The control group combines LucSh-transduced and non-transduced cells, CADM1 downregulated group combines Sh5- and Shm-transduced cells. All data were expressed as a percentage of the levels in the SP-overexpressing cell group. B and C. Scatter plots for the data presented in A with correlation or regression model parameters are shown for Kit ( B ) and F-actin ( C ) as a function of CADM1. Data for SP4- and SP1-expressing cells are shown in different colours. D. Western blotting of protein extracts from LucSh-, SP4- and Shm-transduced HMC-1 cells, with 3 independent transductions for each group, developed with Abs shown on the right of the blots. E. Protein bands, shown in D and Fig. S 2C , were quantified. Bands in SP4 (n = 5) and Shm (n = 4) groups were expressed as percentages of control LucSh/GFP group (n = 5) for each protein, except phosphotyrosine 58-63 kDa bands (n = 3). * P