ABIN307100

antibody from antibodies-online

Targeting: KIT C-Kit, CD117, PBT, SCFR

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN307100

Provider

antibodies-online

Product name

anti-Mast/stem Cell Growth Factor Receptor (KIT) (pTyr721) antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Epitope

pTyr721

Isotype

IgG

Antibody clone number

Polyclonal

Vial size

100 μL

Storage

-20°C

References

Submitted references

Distinct role for c-kit receptor tyrosine kinase and SgIGSF adhesion molecule in attachment of mast cells to fibroblasts.

Koma Y, Ito A, Watabe K, Hirata T, Mizuki M, Yokozaki H, Kitamura T, Kanakura Y, Kitamura Y
Laboratory investigation; a journal of technical methods and pathology 2005 Mar;85(3):426-35

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Anti-apoptotic action of stem cell factor on oocytes in primordial follicles and its signal transduction.

Jin X, Han CS, Yu FQ, Wei P, Hu ZY, Liu YX
Molecular reproduction and development 2005 Jan;70(1):82-90

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Synergistic growth of stem cell factor and granulocyte macrophage colony-stimulating factor involves kinase-dependent and -independent contributions from c-Kit.

Lennartsson J, Shivakrupa R, Linnekin D
The Journal of biological chemistry 2004 Oct 22;279(43):44544-53

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Necessity of tyrosine 719 and phosphatidylinositol 3'-kinase-mediated signal pathway in constitutive activation and oncogenic potential of c-kit receptor tyrosine kinase with the Asp814Val mutation.

Hashimoto K, Matsumura I, Tsujimura T, Kim DK, Ogihara H, Ikeda H, Ueda S, Mizuki M, Sugahara H, Shibayama H, Kitamura Y, Kanakura Y
Blood 2003 Feb 1;101(3):1094-102

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Structure of a c-kit product complex reveals the basis for kinase transactivation.

Mol CD, Lim KB, Sridhar V, Zou H, Chien EY, Sang BC, Nowakowski J, Kassel DB, Cronin CN, McRee DE
The Journal of biological chemistry 2003 Aug 22;278(34):31461-4

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Differential stimulation of c-Kit mutants by membrane-bound and soluble Steel Factor correlates with leukemic potential.

Gommerman JL, Sittaro D, Klebasz NZ, Williams DA, Berger SA
Blood 2000 Dec 1;96(12):3734-42

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Kit signaling through PI 3-kinase and Src kinase pathways: an essential role for Rac1 and JNK activation in mast cell proliferation.

Timokhina I, Kissel H, Stella G, Besmer P
The EMBO journal 1998 Nov 2;17(21):6250-62

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Peptide Competition and Stimulation Extracts prepared from Mo7e cells left untreated (1) or treated with 100 ng/mL human SCF for 5 minutes (2 -5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer overnight at 4oC, then incubated with the c-Kit [pY721] antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non- phosphopeptide corresponding to the phosphopeptide immunogen (3), a generic phosphotyrosine- containing peptide (4), or the