Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- ABIN307101 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Mast/stem Cell Growth Factor Receptor (KIT) (pTyr936) antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- pTyr936
- Isotype
- IgG
- Antibody clone number
- Polyclonal
- Vial size
- 10 miniblots
- Storage
- -20°C
Submitted references Targeting c-kit mutations in solid tumors: scientific rationale and novel therapeutic options.
Identification of Tyr-703 and Tyr-936 as the primary association sites for Grb2 and Grb7 in the c-Kit/stem cell factor receptor.
Demetri GD
Seminars in oncology 2001 Oct;28(5 Suppl 17):19-26
Seminars in oncology 2001 Oct;28(5 Suppl 17):19-26
Identification of Tyr-703 and Tyr-936 as the primary association sites for Grb2 and Grb7 in the c-Kit/stem cell factor receptor.
Thömmes K, Lennartsson J, Carlberg M, Rönnstrand L
The Biochemical journal 1999 Jul 1;341 ( Pt 1):211-6
The Biochemical journal 1999 Jul 1;341 ( Pt 1):211-6
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Supportive validation
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- antibodies-online (provider)
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- Experimental details
- Peptide Competition Extracts prepared from M07e cells left untreated (1) or treated (2-5) with SCF were resolved by SDS- PAGE l and transferred to PVDF. Membranes were blocked with a BSA-TBST buffer overnight incubated with c-Kit [pY936] antibody for two hours at RTr, following prior incubation with: no peptide (1, 2), the non- phosphopeptide corresponding to the immunogen (3), a generic phosphotyrosine-containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab?)2 anti-rabbit IgG alkaline phosphatase and detected using the Human, (b)