Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
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Validation data
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- Product number
- PA5-54750 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ALDH7A1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: DLSLVVPSAL FAAVGTAGQR CTTARRLFIH ESIHDEVVNR LKKAYAQIRV GNPWDPNVLY GPLHTKQAVS MFLGAVEEAK KEGGTVVYGG KVMDRPGNYV EPTIVTGLGH DASIAHTETF APILY Highest antigen sequence identity to the following orthologs: Mouse - 86%, Rat - 89%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ALDH7A1 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human plasma (IgG/HSA depleted); Lane 5: Human liver tissue. Samples were probed using an ALDH7A1 Polyclonal Antibody (Product # PA5-54750).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of ALDH7A1 in human cell line A-431 shows positivity in mitochondria. Samples were probed using an ALDH7A1 Polyclonal Antibody (Product # PA5-54750).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ALDH7A1 in human kidney using an ALDH7A1 Polyclonal Antibody (Product # PA5-54750) shows strong cytoplasmic positivity with a granular pattern in cells in tubules.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ALDH7A1 in human liver using ALDH7A1 Polyclonal Antibody (Product # PA5-54750) shows moderate positivity in cytoplasm granular in hepatocytes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ALDH7A1 in human cerebral cortex using ALDH7A1 Polyclonal Antibody (Product # PA5-54750) shows moderate membranous positivity in microglia.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ALDH7A1 in human skeletal muscle using ALDH7A1 Polyclonal Antibody (Product # PA5-54750) shows no positivity in myocytes as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ALDH7A1 in human kidney using ALDH7A1 Polyclonal Antibody (Product # PA5-54750) shows moderate granular cytoplasmic positivity in cells in tubules.