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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [3]
- Immunohistochemistry [1]
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- Product number
- PA5-29974 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ALDH7A1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: A431, H1299, HeLa, HepG2, mouse liver, rat liver. Predicted reactivity: Human (99%), Mouse (91%), Rat (92%), Zebrafish (82%), Xenopus laevis (81%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μL
- Concentration
- 0.98 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Ataxin-2 (Atxn2)-Knock-Out Mice Show Branched Chain Amino Acids and Fatty Acids Pathway Alterations.
Meierhofer D, Halbach M, Şen NE, Gispert S, Auburger G
Molecular & cellular proteomics : MCP 2016 May;15(5):1728-39
Molecular & cellular proteomics : MCP 2016 May;15(5):1728-39
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ALDH7A1 showing staining in the mitochondria and nucleus of HeLa cells. HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min and stained using an ALDH7A1 polyclonal antibody (Product # PA5-29974) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ALDH7A1 Polyclonal Antibody [C1C3] detects ALDH7A1 protein at mitochondria and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37&#deg;C for 30 min. Green: ALDH7A1 protein stained by ALDH7A1 Polyclonal Antibody [C1C3] (Product # PA5-29974) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ALDH7A1 Polyclonal Antibody [C1C3] detects ALDH7A1 protein at mitochondria and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37&#deg;C for 30 min. Green: ALDH7A1 protein stained by ALDH7A1 Polyclonal Antibody [C1C3] (Product # PA5-29974) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ALDH7A1 Polyclonal Antibody detects ALDH7A1 protein at cytosol on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ALDH7A1 Polyclonal Antibody (Product # PA5-29974) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
