Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ABIN359244 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Branched Chain Ketoacid Dehydrogenase Kinase (BCKDK) (Middle Region) antibody
- Antibody type
- Polyclonal
- Antigen
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the central region of human BCKDK.
- Description
- Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- Middle Region
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Solution structure and dynamics of the lipoic acid-bearing domain of human mitochondrial branched-chain alpha-keto acid dehydrogenase complex.
Branched-chain alpha-ketoacid dehydrogenase kinase. Molecular cloning, expression, and sequence similarity with histidine protein kinases.
Chang CF, Chou HT, Chuang JL, Chuang DT, Huang TH
The Journal of biological chemistry 2002 May 3;277(18):15865-73
The Journal of biological chemistry 2002 May 3;277(18):15865-73
Branched-chain alpha-ketoacid dehydrogenase kinase. Molecular cloning, expression, and sequence similarity with histidine protein kinases.
Popov KM, Zhao Y, Shimomura Y, Kuntz MJ, Harris RA
The Journal of biological chemistry 1992 Jul 5;267(19):13127-30
The Journal of biological chemistry 1992 Jul 5;267(19):13127-30
No comments: Submit comment
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of BCKDK (arrow) using rabbit polyclonal BCKDK Antibody
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.