Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-28050 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BCKDK Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: MCF-7, MCF-7 membrane extract, Mouse brain, rat brain, rat kidney.
- Concentration
- 0.35 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BCKDK using 30 µg of A) HeLa S3 and B) Raji lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a BCKDK polyclonal antibody (Product # PA5-28050) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using BCKDK Polyclonal Antibody (Product # PA5-28050). Sample (20 µg of whole cell lysate). Lane A: mouse brain. 10% SDS PAGE. BCKDK Polyclonal Antibody (Product # PA5-28050) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using BCKDK Polyclonal Antibody (Product # PA5-28050). MCF-7 whole cell and membrane extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK Polyclonal Antibody (Product # PA5-28050) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BCKDK was performed by separating 50 µg of rat tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a BCKDK Polyclonal Antibody (Product # PA5-28050) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BCKDK was performed by separating 50 µg of rat tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a BCKDK Polyclonal Antibody (Product # PA5-28050) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BCKDK Polyclonal Antibody detects BCKDK protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BCKDK stained by BCKDK Polyclonal Antibody (Product # PA5-28050) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin Polyclonal Antibody [GT114] (Product # MA5-31466) diluted at 1:1,000. Blue: Fluoroshield with DAPI .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using BCKDK (Product # PA5-28050) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
