PA5-28050

antibody from Invitrogen Antibodies

Targeting: BCKDK

Antibody data

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Validation data

Product number: PA5-28050 - Provider product page
Provider: Invitrogen Antibodies
Product name: BCKDK Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: MCF-7, MCF-7 membrane extract, Mouse brain, rat brain, rat kidney. Predicted reactivity: Mouse (99%), Rat (100%), Zebrafish (82%), Xenopus laevis (87%), Rhesus Monkey (98%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.35 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

BCKDK protein structure - PA5-28050 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of BCKDK using 30 µg of A) HeLa S3 and B) Raji lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a BCKDK polyclonal antibody (Product # PA5-28050) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using BCKDK Polyclonal Antibody (Product # PA5-28050). Sample (20 µg of whole cell lysate). Lane A: mouse brain. 10% SDS PAGE. BCKDK Polyclonal Antibody (Product # PA5-28050) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using BCKDK Polyclonal Antibody (Product # PA5-28050). MCF-7 whole cell and membrane extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK Polyclonal Antibody (Product # PA5-28050) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of BCKDK was performed by separating 50 µg of rat tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a BCKDK Polyclonal Antibody (Product # PA5-28050) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of BCKDK was performed by separating 50 µg of rat tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a BCKDK Polyclonal Antibody (Product # PA5-28050) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.