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Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- AMAb90841 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#AMAb90841, RRID:AB_2665685
- Product name
- Anti-BRD4
- Antibody type
- Monoclonal
- Description
- Monoclonal Antibody against Human BRD4, Clone ID: CL1115, Gene description: bromodomain containing 4, Alternative Gene Names: CAP, HUNK1, HUNKI, MCAP, Validated applications: WB, IHC, ICC, Uniprot ID: O60885, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- CL1115
- Vial size
- 100 µl
- Concentration
- 1.0 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Notch1 Phase Separation Coupled Percolation facilitates target gene expression and enhancer looping.
Cohesin regulates alternative splicing
AT-hook DNA-binding motif-containing protein one knockdown downregulates EWS-FLI1 transcriptional activity in Ewing's sarcoma cells.
Profiling of diverse tumor types establishes the broad utility of VHL-based ProTaCs and triages candidate ubiquitin ligases.
Foran G, Hallam RD, Megaly M, Turgambayeva A, Antfolk D, Li Y, Luca VC, Necakov A
bioRxiv : the preprint server for biology 2024 Aug 1;
bioRxiv : the preprint server for biology 2024 Aug 1;
Cohesin regulates alternative splicing
Singh A, Chen Q, Nguyen C, Meerzaman D, Singer D
Science Advances 2023;9(9)
Science Advances 2023;9(9)
AT-hook DNA-binding motif-containing protein one knockdown downregulates EWS-FLI1 transcriptional activity in Ewing's sarcoma cells.
Kitagawa T, Kobayashi D, Baron B, Okita H, Miyamoto T, Takai R, Paudel D, Ohta T, Asaoka Y, Tokunaga M, Nakagawa K, Furutani-Seiki M, Araki N, Kuramitsu Y, Kobayashi M
PloS one 2022;17(10):e0269077
PloS one 2022;17(10):e0269077
Profiling of diverse tumor types establishes the broad utility of VHL-based ProTaCs and triages candidate ubiquitin ligases.
Luo X, Archibeque I, Dellamaggiore K, Smither K, Homann O, Lipford JR, Mohl D
iScience 2022 Mar 18;25(3):103985
iScience 2022 Mar 18;25(3):103985
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-BRD4 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of MCF-7 cells using the Anti-BRD4 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
- Sample type
- Human
