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Immunoprecipitation
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Other assay [1]
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- Product number
- 730007 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-BRD4 Monoclonal Antibody (23476)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 23476
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Submitted references Assessment of a method to characterize antibody selectivity and specificity for use in immunoprecipitation.
BioID identifies novel c-MYC interacting partners in cultured cells and xenograft tumors.
Marcon E, Jain H, Bhattacharya A, Guo H, Phanse S, Pu S, Byram G, Collins BC, Dowdell E, Fenner M, Guo X, Hutchinson A, Kennedy JJ, Krastins B, Larsen B, Lin ZY, Lopez MF, Loppnau P, Miersch S, Nguyen T, Olsen JB, Paduch M, Ravichandran M, Seitova A, Vadali G, Vogelsang MS, Whiteaker JR, Zhong G, Zhong N, Zhao L, Aebersold R, Arrowsmith CH, Emili A, Frappier L, Gingras AC, Gstaiger M, Paulovich AG, Koide S, Kossiakoff AA, Sidhu SS, Wodak SJ, Gräslund S, Greenblatt JF, Edwards AM
Nature methods 2015 Aug;12(8):725-31
Nature methods 2015 Aug;12(8):725-31
BioID identifies novel c-MYC interacting partners in cultured cells and xenograft tumors.
Dingar D, Kalkat M, Chan PK, Srikumar T, Bailey SD, Tu WB, Coyaud E, Ponzielli R, Kolyar M, Jurisica I, Huang A, Lupien M, Penn LZ, Raught B
Journal of proteomics 2015 Apr 6;118:95-111
Journal of proteomics 2015 Apr 6;118:95-111
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Immunoprecipitation analysis using Anti-BRD4 Recombinant Mouse Monoclonal Antibody (Product # 730007) followed by western blot detection. Immunoprecipitation was performed on HEK293 cells over expressing flag-tagged BRD4 protein. Western blot using an anti-flag antibody was performed on the raw lysate (lane 1) and immunoprecipitated protein (lane 2) to evaluate the results of Immunoprecipitation. Because detection utilizes an anti-flag antibody, the presence of multiple bands suggests multiple forms of flag-tagged BRD4 protein rather than cross-reactivity of the Anti-BRD4 Recombinant Mouse Monoclonal Antibody.
