PAB29479

antibody from Abnova Corporation

Targeting: BRD4 CAP, HUNK1, HUNKI, MCAP

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29479

Provider

Abnova Corporation

Product name

BRD4 polyclonal antibody

Antibody type

Polyclonal

Description

BRD4 polyclonal antibody raised against recombinant human BRD4.

Antigen sequence

PPFIATQVPVLEPQLPGSVFDPIGHFTQPILHLPQ
PELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALP
QQPSRPSN


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of PC-3 cells with BRD4 polyclonal antibody (Cat# PAB29479) under 1-4 ug/mL working concentration shows positivity in nucleus but excluded from the nucleoli. Antibody staining is shown in green.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human skin with BRD4 polyclonal antibody (Cat# PAB29479) shows strong nuclear positivity in epidermal cells at 1:200 - 1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)