Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoelectron microscopy [1]
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Validation data
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- Product number
- 683004 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#683004, RRID:AB_2572023
- Product name
- Alexa Fluor® 594 anti-BRD4
- Antibody type
- Monoclonal
- Antigen
- Synthetic BRD4 peptide conjugated to KLH (IRSEPFSPSLRPEPPKHC)
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Antibody clone number
- 5C10D05
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- HeLa cells were fixed with 2% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for five minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2 µg/ml BRD4 (clone 5C10D05) Alexa Fluor® 594 (red) in 5% FBS overnight at 4°C. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
- Conjugate
- Yellow dye