STJ11101416

antibody from St John's Laboratory

Targeting: BRD4 CAP, HUNK1, HUNKI, MCAP

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

STJ11101416

Provider

St John's Laboratory

Product name

Anti-BRD4 antibody [ARC0699] (STJ11101416)

Antibody type

Monoclonal

Description

Rabbit monoclonal antibody anti-Brd4 is suitable for use in Western Blot and Immunohistochemistry.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

NA


Epitope

NA

Isotype

IgG

Antibody clone number

NA

Vial size

NA

Concentration

NA

Storage

Store in a freezer at-20°C and avoid freeze-thaw cycles.

Handling

NA

Western blot

Supportive validation

Submitted by

St John's Laboratory (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using BRD4 rabbit monoclonal antibody (STJ11101416) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Sample type

NA

Validation comment

NA

Primary Ab dilution

NA

Other comments

NA

Secondary Ab

NA

Secondary Ab dilution

NA

Protocol

NA

Appendix

NA

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Immunohistochemistry

Supportive validation

Submitted by

St John's Laboratory (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon using BRD4 rabbit monoclonal antibody (STJ11101416) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Sample type

NA

Validation comment

NA

Primary Ab dilution

NA

Other comments

NA

Secondary Ab

NA

Secondary Ab dilution

NA

Protocol

NA

Appendix

NA

Show more