Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [9]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP1-86640 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-86640, RRID:AB_11036484
- Product name
- Rabbit Polyclonal BRD4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human BRD4 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Chromatin remodelling and autocrine TNFα are required for optimal interleukin-6 expression in activated human neutrophils.
RNAi screen identifies Brd4 as a therapeutic target in acute myeloid leukaemia.
Zimmermann M, Aguilera FB, Castellucci M, Rossato M, Costa S, Lunardi C, Ostuni R, Girolomoni G, Natoli G, Bazzoni F, Tamassia N, Cassatella MA
Nature communications 2015 Jan 23;6:6061
Nature communications 2015 Jan 23;6:6061
RNAi screen identifies Brd4 as a therapeutic target in acute myeloid leukaemia.
Zuber J, Shi J, Wang E, Rappaport AR, Herrmann H, Sison EA, Magoon D, Qi J, Blatt K, Wunderlich M, Taylor MJ, Johns C, Chicas A, Mulloy JC, Kogan SC, Brown P, Valent P, Bradner JE, Lowe SW, Vakoc CR
Nature 2011 Aug 3;478(7370):524-8
Nature 2011 Aug 3;478(7370):524-8
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: BRD4 Antibody [NBP1-86640] - Simple Western lane view shows a specific band for BRD4 in 0.2 mg/ml of U-251MG sp lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: BRD4 Antibody [NBP1-86640] - Electropherogram image(s) of corresponding Simple Western lane view. BRD4 antibody was used at 1:60 dilution on U-251MG sp lysates(s).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-86640] - Analysis in human placenta and pancreas tissues. Corresponding BRD4 RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-86640] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-86640] - Staining of human testis shows weak nuclear positivity in a subset of cells in seminiferous ducts.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-86640] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-86640] - Staining of human lymph node shows weak nuclear positivity in lymhocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-86640] - Staining of human tonsil shows moderate to strong nuclear positivity in non-germinal center cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-86640] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-86640] - Staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-86640] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.