Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB100-56487 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56487, RRID:AB_2274853
- Product name
- Rabbit Polyclonal BRD4 Antibody
- Antibody type
- Polyclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: BRD4 Antibody [NB100-56487] - Image shows a specific band for BRD4 in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BRD4 Antibody [NB100-56487] - Analysis of Brd4 in human HeLa lysate in the 1) absence and 2) presence of immunizing peptide and C) mouse NIH 3T3 lysate using Brd4 antibody at 3 ug/mL. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: BRD4 Antibody [NB100-56487] - BRD4 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).