MA5-32016
antibody from Invitrogen Antibodies
Targeting: IGF2R
CD222, CI-M6PR, CI-MPR, CIMPR, M6P-R, MPR1, MPR300, MPRI
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [4]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MA5-32016 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IGF2R Recombinant Rabbit Monoclonal Antibody (SR45-09)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- SR45-09
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IGF2R in different lysates using a Monoclonal antibody (Product #MA5-32016) at a dilution of 1:1,000. Positive control: Lane 1: HepG2, Lane 2: SW480.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-IGF2R Recombinant Rabbit Monoclonal Antibody (SR45-09) (Product # MA5-32016) and a 274 kDa band corresponding to IGF2R was observed across positive cell lines HepG2, PANC-1, Jurkat, HeLa and SW480, low expression in A549 and no expression in HEL 92.1.7. Membrane enriched extracts (30 µg lysate) of Hep G2 (Lane 1), PANC-1 (Lane 2), Jurkat (Lane 3), HeLa (Lane 4), HEL 92.1.7 (Lane 5), SW480 (Lane 6) and A549 (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:10000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-IGF2R Recombinant Rabbit Monoclonal Antibody (SR45-09) (Product # MA5-32016) and a 274 kDa band corresponding to IGF2R was observed across positive cell lines HepG2, PANC-1, Jurkat, HeLa and SW480, low expression in A549 and no expression in HEL 92.1.7. Membrane enriched extracts (30 µg lysate) of Hep G2 (Lane 1), PANC-1 (Lane 2), Jurkat (Lane 3), HeLa (Lane 4), HEL 92.1.7 (Lane 5), SW480 (Lane 6) and A549 (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:10000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of IGF2R in Hela cells using a IGF2R Monoclonal antibody (Product # MA5-32016) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of IGF2R in MCF-7 cells using a IGF2R Monoclonal antibody (Product # MA5-32016) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of IGF2R in HepG2 cells using a IGF2R Monoclonal antibody (Product # MA5-32016) as seen in green. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of IGF2R was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with IGF2R Recombinant Rabbit Monoclonal Antibody (SR45-09) (Product # MA5-32016) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Alexa Fluor™ 647 Phalloidin (Product #A22287, 1:300). Panel d represents the merged image showing Golgi and cytoplasmic localization. Panel e represents low expressing cell line HEL 92.1.7. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of IGF2R of paraffin-embedded Human tonsil tissue using a IGF2R Monoclonal antibody (Product #MA5-32016). Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of IGF2R in Hela cells using a IGF2R Monoclonal Antibody (Product # MA5-32016) at a dilution of 1:50, as seen in blue compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.