PA5-110100

antibody from Invitrogen Antibodies

Targeting: PFDN4 C-1, C1, PFD4

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-110100

Provider

Invitrogen Antibodies

Product name

PFDN4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MAATMKKAAA EDVNVTFEDQ QKINKFARNT SRITELKEEI EVKKKQLQNL EDACDDIMLA DDDCLMIPYQ IGDVFISHSQ EETQEMLEEA KKNLQEEIDA LESRVESIQR VLADLKVQLY AKFGSNINLE ADES

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 μL

Concentration

0.86 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PFDN4 in extracts of various cell lines. Samples were incubated with PFDN4 Polyclonal antibody (Product # PA5-110100) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of PFDN4 in U-2OS cells. Samples were incubated in polyclonal PFDN4 antibody (Product # PA5-110100) using a dilution of 1:100, followed by DAPI.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of PFDN4 in C6 cells. Samples were incubated in polyclonal PFDN4 antibody (Product # PA5-110100) using a dilution of 1:100, followed by DAPI.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of PFDN4 in L929 cells. Samples were incubated in polyclonal PFDN4 antibody (Product # PA5-110100) using a dilution of 1:100, followed by DAPI.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of PFDN4 in U-2OS cells. Samples were incubated in polyclonal PFDN4 antibody (Product # PA5-110100) using a dilution of 1:100, followed by DAPI.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of PFDN4 in C6 cells. Samples were incubated with PFDN4 Polyclonal antibody (Product # PA5-110100) using a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of PFDN4 in L929 cells. Samples were incubated with PFDN4 Polyclonal antibody (Product # PA5-110100) using a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of PFDN4 in U-2 OS cells. Samples were incubated with PFDN4 Polyclonal antibody (Product # PA5-110100) using a dilution of 1:100. Blue: DAPI for nuclear staining.