MA5-42788

antibody from Invitrogen Antibodies

Targeting: KRT2 KRT2A, KRTE

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-42788

Provider

Invitrogen Antibodies

Product name

Cytokeratin 2e Recombinant Rabbit Monoclonal Antibody (9T3Q2)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive test controls include: A-431. Immunogen sequence: YMIKVELQSK VDLLNQEIEF LKVLYDAEIS QIHQSVTDTN VILSMDNSRN LDLDSIIAEV KAQYEEIAQR SKEEAEALYH SKYEELQVTV GRHGDSLKEI KIEISELNRV IQRLQGEIAH VKKQCKNVQD

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

9T3Q2

Vial size

100 µL

Concentration

0.3 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Cytokeratin 2e in A-431 cells using a Cytokeratin 2e Recombinant Monoclonal antibody (Product # MA5-42788) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 180s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of Cytokeratin 2e in human skin using a Cytokeratin 2e Recombinant Monoclonal antibody (Product # MA5-42788). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of Cytokeratin 2e in human skin using a Cytokeratin 2e Recombinant Monoclonal antibody (Product # MA5-42788). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of Cytokeratin 2e in human skin using a Cytokeratin 2e Recombinant Monoclonal antibody (Product # MA5-42788). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of Cytokeratin 2e in human skin using a Cytokeratin 2e Recombinant Monoclonal antibody (Product # MA5-42788). Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Cytokeratin 2e in mouse skin using a Recombinant Monoclonal antibody (Product # MA5-42788) at a dilution of 1:100. Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol..