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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [6]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- 13615-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IFT20 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.22 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 13615-1-AP (IFT20 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of IFT20 antibody (13615-1-AP, 1:500) with si-Control and si-IFT20 transfected HEK293 cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13615-1-AP (IFT20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MDCK cells were subjected to SDS PAGE followed by western blot with 13615-1-AP (IFT20 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 13615-1-AP (IFT20 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse testis tissue were subjected to SDS PAGE followed by western blot with 13615-1-AP (IFT20 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IF result (localization to Golgi-like structures) of anti-IFT20 (13615-1-AP, 1:50) with serum-starved hTERT-RPE1 (MoTH fixed) by Dr. Moshe Kim.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (10% Formaldehyde) fixed MDCK cells using 13615-1-AP (IFT20 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human endometrial cancer tissue slide using 13615-1-AP ( IFT20 Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human endometrial cancer tissue slide using 13615-1-AP ( IFT20 Antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-IFT20 (IP:13615-1-AP, 3ug; Detection:13615-1-AP 1:700) with mouse testis tissue lysate 4000ug.
