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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Other assay [1]
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- Product number
- PA5-92051 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RhoGAP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: VLKYDDFLKS TQKSPATAPK PMPPRPPLPN QQFGVSLQHL QEKNPEQEPI PIVLRETVAY LQAHALTTEG IFRRSANTQV VREVQQKYNM GLPVDFDQYN ELHLPAVILK TFLRELPEPL LTFDLYPHVV GFLNIDESQR VPATLQVLQT LPEENYQVLR FLTAFLVQIS AHSDQNKMTN TNLAVVFGPN LLWAKDAAIT LKAINPINTF TKFLLDHQGE LFPSPDPSGL; Positive Samples: BxPC-3, HeLa, HepG2, U-87MG, PC-12, Mouse lung; Cellular Location: Cytoplasm
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.42 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RhoGAP in extracts of various cell lines. Samples were incubated with RhoGAP Polyclonal antibody (Product # PA5-92051) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of RhoGAP was performed in HeLa cells using RhoGAP Polyclonal Antibody (Product # PA5-92051).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of RhoGAP in 200 μg extracts of HeLa cells. Samples were precipitated with 3 μg RhoGAP Polyclonal antibody (Product # PA5-92051). Western blot was performed from the immunoprecipitate using RhoGAP Polyclonal antibody (Product # PA5-92051) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of RhoGAP was performed in 200 µg extracts of HeLa cells using RhoGAP Polyclonal Antibody (Product # PA5-92051). Western blot was performed from the immunoprecipitate using RhoGAP Polyclonal Antibody.
