PA5-27426

antibody from Invitrogen Antibodies

Targeting: ARHGAP1 CDC42GAP, p50rhoGAP, RhoGAP

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27426

Provider

Invitrogen Antibodies

Product name

RhoGAP Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, HeLa, HepG2, Mouse brain, ARHGAP1-transfected 293T. Predicted reactivity: Mouse (95%), Rat (92%), Dog (96%), Rhesus Monkey (98%), Bovine (92%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

miR-130b directly targets ARHGAP1 to drive activation of a metastatic CDC42-PAK1-AP1 positive feedback loop in Ewing sarcoma.

Satterfield L, Shuck R, Kurenbekova L, Allen-Rhoades W, Edwards D, Huang S, Rajapakshe K, Coarfa C, Donehower LA, Yustein JT
International journal of cancer 2017 Nov 15;141(10):2062-2075

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ARHGAP1 using 30 µg of A431 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an ARHGAP1 polyclonal antibody (Product # PA5-27426) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of RhoGAP was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a RhoGAP Polyclonal Antibody (Product # PA5-27426) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using RhoGAP Polyclonal Antibody (Product # PA5-27426). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 10% SDS PAGE. RhoGAP Polyclonal Antibody (Product # PA5-27426) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of RhoGAP was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a RhoGAP Polyclonal Antibody (Product # PA5-27426) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

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