A305-758A-T

antibody from Invitrogen Antibodies

Targeting: NEMF FLJ10051, NY-CO-1, SDCCAG1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A305-758A-T

Provider

Invitrogen Antibodies

Product name

NEMF Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

10 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human NEMF by western blot. Samples: Whole cell lysate (10 µg) from Jurkat, HeLa, HEK293T, RKO, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NEMF antibody (Product # A305-758A lot 2) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human NEMF by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NEMF antibody A305-758A-M (lot A305-758A-M-1) used for IP at 50 µl per reaction. NEMF was also immunoprecipitated by rabbit anti-NEMF antibody A305-757A-M. For blotting immunoprecipitated NEMF, A305-758A-M was used at 1:100. Detection: Chemiluminescence with an exposure time of 3 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human NEMF by western blot. Samples: Whole cell lysate (15 µg) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NEMF antibody A305-758A-M (lot A305-758A-M-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human NEMF by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NEMF antibody (Product # A305-758A; Lot 2) used for IP at 6 µg per reaction. NEMF was also immunoprecipitated by a previous lot of this antibody (Product # A305-758A; lot 2) and a second antibody against a different epitope of NEMF (Product # A305-757A). For blotting immunoprecipitated NEMF, Product # A305-758A was used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.