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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- PA5-87956 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MEGF10 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Samples: U-251MG, HT-29, MCF7, Mouse brain, Mouse liver, Rat brain; Cellular Location: Basolateral cell membrane, Cell membrane, Cell projection, Single-pass type I membrane protein, phagocytic cup Immunogen sequence: ADYTISGTLP HSNGGNANSH YFTNPSYHTL TQCATSPHVN NRDRMTVTKS KNNQLFVNLK NVNPGKRGPV GDCTGTLPAD WKHGGYLNEL GAFGLDRSYM GKSLKDLGKN SEYNSSNCSL SSSENPYATI KDPPV
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western Blot analysis of MEGF10 in extracts of various cell lines using MEGF10 Polyclonal Antibody (Product # PA5-87956) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
