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- Antibody Data
- Antigen structure
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- Product number
- PA5-101660 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GSDMC Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Antibody detects endogenous levels of total GSDMC.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Molecular Characteristics, Clinical Implication, and Cancer Immunity Interactions of Pyroptosis-Related Genes in Breast Cancer.
Xu D, Ji Z, Qiang L
Frontiers in medicine 2021;8:702638
Frontiers in medicine 2021;8:702638
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GSDMC in HepG2 cell lysate (left lane: treated with blocking peptide). Samples were incubated with GSDMC polyclonal antibody (Product # PA5-101660).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of GSDMC in 293 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with GSDMC polyclonal antibody (Product # PA5-101660) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of GSDMC in HeLa cells. Samples were incubated with GSDMC polyclonal antibody (Product # PA5-101660).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 9 Validation of the expression of GSDMC, IL-18 , and TIRAP in normal breast cells (MCF-10A) and breast cancer cells (MDA-MB-231 and HCC70). (A-D) Western blot detecting the expression of GSDMC, IL-18, and TIRAP in MCF-10A, MDA-MB-231, and HCC70 cells. *** p < 0.001; **** p < 0.0001 . (E-G) Immunofluorescence of the expression of GSDMC, IL-18, and TIRAP in MCF-10A, MDA-MB-231, and HCC70 cells. Scale bar, 5 mum; magnification 200 x.
