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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- ELISA [1]
- Immunoprecipitation [1]
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- Product number
- H00493829-M05 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00493829-M05, RRID:AB_1577804
- Product name
- TRIM72 monoclonal antibody (M05), clone 3B5
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant LOC493829.
- Antigen sequence
QGLWLLGLREGKILEAHVEAKEPRALRSPERRPTR
IGLYLSFGDGVLSFYDASDADALVPLFAFHERLPR
PVYPFFDVCWHDKGKNAQPLLLVGPEGAEA- Isotype
- IgG
- Antibody clone number
- 3B5
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged TRIM72 is 0.1 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of TRIM72 transfected lysate using anti-TRIM72 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with TRIM72 MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
