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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
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- Product number
- LS-C312888 - Provider product page
- Provider
- LSBio
- Product name
- SLC2A5 / GLUT5 Antibody (aa232-251) LS-C312888
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified
- Reactivity
- Mouse
- Host
- Rabbit
- Storage
- At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- WB of SLC2A5 / GLUT5 antibody. Lane 1: Rat Kidney Tissue Lysate. Lane 2: Rat liver Tissue Lysate. Lane 3: Mouse Kidney Tissue Lysate.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-SLC2A5 antibody, Western blotting All lanes: Anti SLC2A5 at 0.5ug/ml Lane 1: Rat Kidney Tissue Lysate at 50ugLane 2: Rat Liver Tissue Lysate at 50ugLane 3: Mouse Kidney Tissue Lysate at 50ugPredicted bind size: 55KD Observed bind size: 55KD
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- SLC2A5 / GLUT5 antibody. IHC(P): Rat Kidney Gland Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- SLC2A5 / GLUT5 antibody. IHC(F): Rat Intestine Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC analysis of GLUT5 using anti-GLUT5 antibody. GLUT5 was detected in paraffin-embedded section of mouse ggj3249 tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-GLUT5 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
