TA500555

antibody from OriGene

Targeting: SLC2A5 GLUT5

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500555

Provider

OriGene

Proper citation

OriGene Cat#TA500555, RRID:AB_10585880

Product name

SLC2A5 (GLUT5) mouse monoclonal antibody, clone OTI9H4 (formerly 9H4)

Antibody type

Monoclonal

Description

SLC2A5 (GLUT5) mouse monoclonal antibody, clone OTI9H4 (formerly 9H4)

Host

Mouse

Conjugate

Unconjugated

Epitope

SLC2A5

Isotype

IgG

Antibody clone number

OTI9H4

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-SLC2A5 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLC2A5 (RC200418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLC2A5.

Validation comment

WB

Immunoprecipitation

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunoprecipitation(IP) of SLC2A5 by using TrueMab monoclonal anti-SLC2A5 antibodies (Negative control: IP without adding anti-SLC2A5 antibody.). For each experiment, 500ul of DDK tagged SLC2A5 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-SLC2A5 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Validation comment

IP