Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA500575 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500575, RRID:AB_10584475
- Product name
- SLC2A5 (GLUT5) mouse monoclonal antibody, clone OTI12F2 (formerly 12F2)
- Antibody type
- Monoclonal
- Description
- SLC2A5 (GLUT5) mouse monoclonal antibody, clone OTI12F2 (formerly 12F2)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SLC2A5
- Isotype
- IgG
- Antibody clone number
- OTI12F2
- Vial size
- 100 µl
- Concentration
- 0.6 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLC2A5 (RC200418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLC2A5.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HT29 cells using anti-SLC2A5 mouse monoclonal antibody (TA500575).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunoprecipitation(IP) of SLC2A5 by using TrueMab monoclonal anti-SLC2A5 antibodies (Negative control: IP without adding anti-SLC2A5 antibody.). For each experiment, 500ul of DDK tagged SLC2A5 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-SLC2A5 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
- Validation comment
- IP
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC200418 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SLC2A5 antibody(TA500575), and then analyzed by flow cytometry.
- Validation comment
- FC
