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- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- PA5-80023 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GLUT5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 500 µg/mL
- Storage
- -20°C
Submitted references GLUT5 (SLC2A5) enables fructose-mediated proliferation independent of ketohexokinase.
Liang RJ, Taylor S, Nahiyaan N, Song J, Murphy CJ, Dantas E, Cheng S, Hsu TW, Ramsamooj S, Grover R, Hwang SK, Ngo B, Cantley LC, Rhee KY, Goncalves MD
Cancer & metabolism 2021 Mar 24;9(1):12
Cancer & metabolism 2021 Mar 24;9(1):12
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of SLC2A5 using anti-SLC2A5 antibody (Product # PA5-80023) . SLC2A5 was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-SLC2A5 antibody (Product # PA5-80023) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry of GLUT5 in THP-1 cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with GLUT5 Polyclonal Antibody (Product # PA5-80023) at a dilution of 1 μg (per 1x10^6 cells), followed by DyLight®488 conjugated goat anti-rabbit IgG (for 30 minutes at 20°C) using 5-10 μg (per 1x10^6 cells) dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 3 GLUT5 overexpression rescues cellular proliferation in fructose. a Normalized expression of genes that are differentially expressed ( q = 0.4, >1.1 log 2 fold change) between PC3 and semi-trained PC3 cells (passage 20) presented in heatmap form. b Relative expression of SLC2A5 transcript in semi-trained PC3 and trained PC3 cells as compared to the parental PC3 line. Two primer sets were used ( n = 2 per condition). Two-way ANOVA with Fisher's LDS test. * P < 0.05, **** P < 0.0001. c Immunoblot of the indicated proteins using lysates from PC3, semi-trained PC3, and trained PC3 cells. The murine liver and muscle used as controls. d GLUT5 or an empty vector (EV) were overexpressed in the indicated cells lines. The cells were plated at 20,000-30,000 cells/well and then grown in the presence of no sugar, 10 mM fructose, or 10 mM glucose. After 3 days, the cells were fixed and stained with crystal violet solution ( n = 2 per condition)
