- PA5-80023 - Invitrogen Antibodies SLC2A5 antibody

Submitted references GLUT5 (SLC2A5) enables fructose-mediated proliferation independent of ketohexokinase.

Liang RJ, Taylor S, Nahiyaan N, Song J, Murphy CJ, Dantas E, Cheng S, Hsu TW, Ramsamooj S, Grover R, Hwang SK, Ngo B, Cantley LC, Rhee KY, Goncalves MD
Cancer & metabolism 2021 Mar 24;9(1):12

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry analysis of SLC2A5 using anti-SLC2A5 antibody (Product # PA5-80023) . SLC2A5 was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-SLC2A5 antibody (Product # PA5-80023) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry analysis of SLC2A5 using anti-SLC2A5 antibody (Product # PA5-80023) . SLC2A5 was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-SLC2A5 antibody (Product # PA5-80023) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of GLUT5 on paraffin-embedded rat intestine cancer tissue. Sample was incubated with GLUT5 polyclonal antibody (Product# PA5-80023) with a dilution of 1 µg/mL, and developed by Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of GLUT5 on paraffin-embedded human intestinal cancer tissue. Sample was incubated with GLUT5 polyclonal antibody (Product# PA5-80023) with a dilution of 1 µg/mL, and developed by Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow Cytometry of GLUT5 in THP-1 cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with GLUT5 Polyclonal Antibody (Product # PA5-80023) at a dilution of 1 μg (per 1x10^6 cells), followed by DyLight®488 conjugated goat anti-rabbit IgG (for 30 minutes at 20°C) using 5-10 μg (per 1x10^6 cells) dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of GLUT5 in THP-1 cells using GLUT5 Polyclonal Antibody (Product # PA5-80023), shown in overlay histogram (blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum, and incubated with the primary antibody (1 μg/1x10^6 cells) for 30 min at 20°C. DyLight 488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow Cytometry of GLUT5 in THP-1 cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with GLUT5 Polyclonal Antibody (Product # PA5-80023) at a dilution of 1 μg (per 1x10^6 cells), followed by DyLight®488 conjugated goat anti-rabbit IgG (for 30 minutes at 20°C) using 5-10 μg (per 1x10^6 cells) dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 3 GLUT5 overexpression rescues cellular proliferation in fructose. a Normalized expression of genes that are differentially expressed ( q = 0.4, >1.1 log 2 fold change) between PC3 and semi-trained PC3 cells (passage 20) presented in heatmap form. b Relative expression of SLC2A5 transcript in semi-trained PC3 and trained PC3 cells as compared to the parental PC3 line. Two primer sets were used ( n = 2 per condition). Two-way ANOVA with Fisher's LDS test. * P < 0.05, **** P < 0.0001. c Immunoblot of the indicated proteins using lysates from PC3, semi-trained PC3, and trained PC3 cells. The murine liver and muscle used as controls. d GLUT5 or an empty vector (EV) were overexpressed in the indicated cells lines. The cells were plated at 20,000-30,000 cells/well and then grown in the presence of no sugar, 10 mM fructose, or 10 mM glucose. After 3 days, the cells were fixed and stained with crystal violet solution ( n = 2 per condition)

PA5-80023

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