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- Antibody Data
- Antigen structure
- References [1]
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- Immunocytochemistry [2]
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- Product number
- PA5-20059 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SARM Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is Daudi cell lysate. PA5-20059 can be used with blocking peptide PEP-0178.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C
Submitted references Implication of Toll/IL-1 receptor domain containing adapters in Porphyromonas gingivalis-induced inflammation.
Bugueno IM, Benkirane-Jessel N, Huck O
Innate immunity 2021 May;27(4):324-342
Innate immunity 2021 May;27(4):324-342
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of Daudi cells using SARM Polyclonal Antibody (Product # PA5-20059) at 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of SARM in Daudi cells with SARM Polyclonal Antibody (Product # PA5-20059) at 2 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4. P. gingivalis induced the activation of TLR2 and TLR4, activation of the SARM adapter, and the inhibition of SOCS-1 protein expression. (A) Protein expression of TLR2, TLR4 in response to P. gingivalis ( Pg ) (MOI:100) for 24 h was evaluated by Western blot. (B) Protein expression of SARM and SOCS-1 in response to P. gingivalis (MOI:100) for 24 h in GEC and EC was evaluated by Western blot. All bands were compared and normalized with beta-actin expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 9. Co-immunoprecipitated proteins using Dynabeads Protein G, identifying interactions between the five TIR domains containing proteins (MyD88, MAL, TRIF, TRAM, SARM) and TLR2 and TLR4 in GEC (A) and EC (B), by SDS-PAGE and blotting of the protein complexes. In each column, the primary precipitated protein by the Ab capture is indicated in the upper part. Then, in each band, the presence and expression of the different proteins that are interacting at the time of precipitation are indicated with the arrows.
