F55031

antibody from NSJ Bioreagents

Targeting: GUSB

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F55031

Provider

NSJ Bioreagents

Product name

Beta Glucuronidase Antibody / Gusb

Antibody type

Polyclonal

Description

This highly specific Beta Glucuronidase antibody is suitable for use in Immunohistochemistry/Flow cytometry/Western blot applications with human and mouse samples.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the Beta Glucuronidase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) HT-29, 2) WiDr, 3) K562, 4) A2058, 5) HL60, 6) mouse liver and 7) mouse lung lysate with Beta Glucuronidase antibody. Expected molecular weight: 68-75 kDa, also seen as dimers and tetramers.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human liver tissue with Beta Glucuronidase antibody. HIER: steam section in pH9 EDTA buffer for 20 min and allow to cool prior to staining.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human colon carcinoma tissue with Beta Glucuronidase antibody. HIER: steam section in pH9 EDTA buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human WiDr cells with Beta Glucuronidase antibody; Blue=isotype control, Green= Beta Glucuronidase antibody.