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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [2]
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- Product number
- RQ5581 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Beta Glucuronidase Antibody / Gusb
- Antibody type
- Polyclonal
- Description
- This highly specific Beta Glucuronidase antibody is suitable for use in Western blot/Immunofluorescence/Immunocytochemistry/Flow cytometry/Direct ELISA applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the Beta Glucuronidase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) K562, 2) Caco-2, 3) U-87 MG and 4) HepG2 cell lysate with Beta Glucuronidase antibody. Expected molecular weight: 68-75 kDa, also seen as dimers and tetramers.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFEP human A431 cells with Beta Glucuronidase antibody (red) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human Caco-2 cells with Beta Glucuronidase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Beta Glucuronidase antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human A431 cells with Beta Glucuronidase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Beta Glucuronidase antibody.
