20341-1-AP

antibody from Proteintech Group

Targeting: APLNR AGTRL1, APJ, APJR, FLJ90771

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

20341-1-AP

Provider

Proteintech Group

Product name

APJ antibody

Antibody type

Polyclonal

Description

APJ antibody (Cat. #20341-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: FC, WB, ELISA.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Neural APLNR Deficiency-Induced Anxiety-Like Behaviors Associate with the Alteration of Global Gene Expression.

Wang X, Zheng H, Chen S, Zhao K, Li M, Cai Z, Bao G, Wu Y, Song W
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2026 Mar 31;40(6):e71678

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Apelin-13 alleviates post-infarction injury by modulating macrophage inflammation via APJ-dependent inhibition of NLRP3-mediated pyroptosis.

Xu XJ, Shen K, Ma YJ, Zhang XL, Wang Q, Liu P, He QF, Wang XQ, Xu YN, Fu HL, Yang KX, Li JJ, Luo ZX
International immunopharmacology 2026 Jul 1;180:116666

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A pan-cancer single-cell transcriptomic atlas of human bone metastases.

Wang S, Ma F, Wang D, Yu Q, Zheng H, Chen Z, Zhao S, Xu L, Ye Q, Tan Y, Huang W, Sun Z, Wu Z, Cai W, Fang M, Cheng M, Ji Y, Zhang Y, Gu B, Song S, Chen Y, Zhang J, Shao Y, Yan W, Sun Y
Cell reports. Medicine 2026 Feb 17;7(2):102583

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Targeting APJ drives BNIP3-PINK1-PARKIN induced mitophagy and improves systemic inflammatory bone loss.

Wang W, Wang Q, Li W, Xu H, Liang X, Wang W, Li N, Yang H, Xu Y, Bai J, Yang S, Geng D
Journal of advanced research 2025 Oct;76:655-668

---

Effects of apelin on age-associated neovascularization impairment in peripheral artery disease.

Chen J, Chen M, Liu Y, Wang X, Yao M, Chen J, Zhang J, Peng Q
Life sciences 2025 Nov 15;381:123980

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Apelin/APJ alleviates diabetic nephropathy by improving glomerular endothelial cells dysfunction via SIRT3‑KLF15.

Huang M, Chang J, Liu Y, Yin J, Zeng X
Molecular medicine reports 2025 May;31(5)

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Apelin Alleviated Endothelial-To-Mesenchymal Transition via Increasing BKCa in Diabetic Nephropathy.

Fan C, Liu Y, Chang J, Huang M, Gao Z, Wang H, Zheng X, Li M, Xu J, Yin J, Zeng X
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2025 Jun 30;39(12):e70747

---

Aerobic Exercise Upregulates Renal-Derived ELABELA to Improve Myocardial Fibrosis by Activating APJ-AMPK-Sirt1 and Inhibiting TGFβ1-Smad2/3 Signaling Pathways in Myocardial Infarction Mice.

Ren W, Xi Y, Ma Y, Duan X, Wang T, Yu M, Qin S, Gong DW, Tian Z
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2025 Jun 30;39(12):e70734

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Plant-derived miR166a-3p packaged into exosomes to cross-kingdom inhibit mammary cell proliferation and promote apoptosis by targeting APLNR gene.

Shi Y, Wei J, Nie Y, Luo J, Chen T, Xi Q, Zhang Y, Sun J
International journal of biological macromolecules 2025 Jan;286:138470

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Apelin/APJ increased renal blood flow through endothelial BKCa channel induced p-eNOS and ET-1 in diabetic conditions.

Huang M, Chang J, Liu Y, Yin J, Zeng X
Peptides 2025 Jan;183:171333

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A reactive oxygen species-responsive hydrogel loaded with Apelin-13 promotes the repair of spinal cord injury by regulating macrophage M1/M2 polarization and neuroinflammation.

Li Z, Zhao Q, Zhou J, Li Y, Zheng Y, Chen L
Journal of nanobiotechnology 2025 Jan 10;23(1):12

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Apelin-13/APJ promotes neural stem cells to repair ischemic stroke.

Li S, Yuan S, Yang S, Zhou C, Zhong Y, Huang Z, Meng C, Pei L, Xie Y, Chen X, Wu H, Guo Y, Long H, Wang T
Tissue & cell 2025 Aug;95:102872

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Single-cell atlas of endothelial and mural cells across primary and metastatic brain tumors.

Bejarano L, Lourenco J, Kauzlaric A, Lamprou E, Costa CF, Galland S, Maas RR, Guerrero Aruffo P, Fournier N, Brouland JP, Hottinger AF, Daniel RT, Hegi ME, Joyce JA
Immunity 2025 Apr 8;58(4):1015-1032.e6

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Apelin-13-Loaded Macrophage Membrane-Encapsulated Nanoparticles for Targeted Ischemic Stroke Therapy via Inhibiting NLRP3 Inflammasome-Mediated Pyroptosis.

Ma CS, Ma YP, Han B, Duan WL, Meng SC, Bai M, Dong H, Zhang LY, Duan MY, Liu J, Deng AJ, He MT
International journal of nanomedicine 2024;19:9175-9193

---

Apelin-13 alleviates intrauterine adhesion by inhibiting epithelial-mesenchymal transition of endometrial epithelial cells and promoting angiogenesis.

Zhao Q, Li Y, Zhao X, Zhou J, Zheng Y, Li Z
Human cell 2024 Nov;37(6):1613-1623

---

The Elabela-APJ axis attenuates sepsis-induced myocardial dysfunction by reducing pyroptosis by balancing the formation and degradation of autophagosomes.

Liu S, Liu FZ, Yan JY, Fang X, Xu ZP, Cai HL, Yang YJ, Yu YW
Free radical biology & medicine 2024 Nov 1;224:405-417

---

APLN promotes the proliferation, migration, and glycolysis of cervical cancer through the PI3K/AKT/mTOR pathway.

Wang Q, Wang B, Zhang W, Zhang T, Liu Q, Jiao X, Ye J, Hao Y, Gao Q, Ma G, Hao C, Cui B
Archives of biochemistry and biophysics 2024 May;755:109983

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Apelin stimulation of the vascular skeletal muscle stem cell niche enhances endogenous repair in dystrophic mice.

Le Moal E, Liu Y, Collerette-Tremblay J, Dumontier S, Fabre P, Molina T, Dort J, Orfi Z, Denault N, Boutin J, Michaud J, Giguère H, Desroches A, Trân K, Ellezam B, Vézina F, Bedard S, Raynaud C, Balg F, Sarret P, Boudreault PL, Scott MS, Denault JB, Marsault E, Feige JN, Auger-Messier M, Dumont NA, Bentzinger CF
Science translational medicine 2024 Mar 20;16(739):eabn8529

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Structure-based design of non-hypertrophic apelin receptor modulator.

Wang WW, Ji SY, Zhang W, Zhang J, Cai C, Hu R, Zang SK, Miao L, Xu H, Chen LN, Yang Z, Guo J, Qin J, Shen DD, Liang P, Zhang Y, Zhang Y
Cell 2024 Mar 14;187(6):1460-1475.e20

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Protective effect of apelin-13 on ventilator-induced acute lung injury.

Lian S, Zhang X, Shen Y, He S, Chen Z, Zhou L, Jiang W
Molecular biology reports 2024 Jan 4;51(1):74

---

Adaptive enzyme-responsive self-assembling multivalent apelin ligands for targeted myocardial infarction therapy.

Li J, Song X, Liao X, Shi Y, Chen H, Xiao Q, Liu F, Zhan J, Cai Y
Journal of controlled release : official journal of the Controlled Release Society 2024 Aug;372:571-586

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Neuroprotective Effect and Mechanism of Tanreqing Injection on Ischemic Stroke: Insights from Network Pharmacology and in vivo Experiments.

Li ZH, Pu XQ, Li SS, Dong XK, Zhang GQ, Wang Y, Liu JM
Chinese journal of integrative medicine 2024 Aug;30(8):713-720

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The regulatory role of the apelin/APJ axis in scarring: Identification of upstream and downstream mechanisms.

Shi N, Wang Y, Xia Z, Zhang J, Jia S, Jiao Y, Wang C, Wang X, Zhao J, Zhang J, Jiang D
Biochimica et biophysica acta. Molecular basis of disease 2024 Apr;1870(4):167125

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Immunological characterization of stroke-heart syndrome and identification of inflammatory therapeutic targets.

Zheng J, Ma Y, Guo X, Wu J
Frontiers in immunology 2023;14:1227104

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Elabela inhibits TRAF1/NF-κB induced oxidative DNA damage to promote diabetic foot ulcer wound healing.

Hong Y, Li J, Zhong Y, Yang S, Pei L, Huang Z, Chen X, Wu H, Zheng G, Zeng C, Wu H, Wang T
iScience 2023 Sep 15;26(9):107601

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Exercise improves cardiac function and attenuates myocardial inflammation and apoptosis by regulating APJ/STAT3 in mice with stroke.

Wang L, Tu W, Li X, Li C, Lu J, Dai P, Chen Y, Gu M, Li M, Jiang S, Yang G, Li S
Life sciences 2023 Nov 1;332:122041

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Exercise improves homeostasis of the intestinal epithelium by activation of apelin receptor-AMP-activated protein kinase signalling.

Chae SA, Du M, Son JS, Zhu MJ
The Journal of physiology 2023 Jun;601(12):2371-2389

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ELABELA/APJ Axis Prevents Diabetic Glomerular Endothelial Injury by Regulating AMPK/NLRP3 Pathway.

Chen Z, Wang Z, Hu Y, Lin H, Yin L, Kong J, Zhang Y, Hu B, Li T, Zheng X, Yang Q, Ye S, Wang S, Zhou Q, Zheng C
Inflammation 2023 Dec;46(6):2343-2358

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Apelin Receptor Can Act as a Specific Marker and Promising Therapeutic Target for Infantile Hemangioma.

Chen Q, Ying H, Yu Z, Chang L, Chen Z, Chen J, Chang SJ, Qiu Y, Lin X
The Journal of investigative dermatology 2023 Apr;143(4):566-577.e12

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Elabela-apelin-12, 17, 36/APJ system promotes platelet aggregation and thrombosis via activating the PANX1-P2X7 signaling pathway.

Chen Z, Luo X, Liu M, Jiang J, Li Y, Huang Z, Wang L, Cao J, He L, Huang S, Hu H, Li L, Chen L
Journal of cellular biochemistry 2023 Apr;124(4):586-605

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The Neuroprotective Effect of Shenmai Injection on Oxidative Stress Injury in PC12 Cells Based on Network Pharmacology.

Wu J, Wu J, Li Z, Dong X, Yuan S, Liu J, Wang L
Evidence-based complementary and alternative medicine : eCAM 2022;2022:6969740

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Apelin-13 Pretreatment Promotes the Cardioprotective Effect of Mesenchymal Stem Cells against Myocardial Infarction by Improving Their Survival.

Chen G, Liang X, Han Q, Mai C, Shi L, Shao Z, Hong Y, Lin F, Li M, Hu B, Li X, Zhang Y
Stem cells international 2022;2022:3742678

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Apelin-13 Attenuates Lipopolysaccharide-Induced Inflammatory Responses and Acute Lung Injury by Regulating PFKFB3-Driven Glycolysis Induced by NOX4-Dependent ROS.

Yuan Y, Wang W, Zhang Y, Hong Q, Huang W, Li L, Xie Z, Chen Y, Li X, Meng Y
Journal of inflammation research 2022;15:2121-2139

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Apelin and Apelin Receptor in Follicular Granulosa Cells of Buffalo Ovaries: Expression and Regulation of Steroidogenesis.

Shokrollahi B, Zheng HY, Li LY, Tang LP, Ma XY, Lu XR, Duan AQ, Zhang Y, Tan XH, Huang CX, Xu YY, Shang JH
Frontiers in endocrinology 2022;13:844360

---

Exosomal miR-22-3p from Mesenchymal Stem Cells Inhibits the Epithelial-Mesenchymal Transition (EMT) of Melanoma Cells by Regulating LGALS1.

Chen Y, Fang Y, Li L, Luo H, Cao T, Tu B
Frontiers in bioscience (Landmark edition) 2022 Sep 29;27(9):275

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The Apelin-APJ axis alleviates LPS-induced pulmonary fibrosis and endothelial mesenchymal transformation in mice by promoting Angiotensin-Converting Enzyme 2.

Wang H, Cong L, Yin X, Zhang N, Zhu M, Sun T, Fan J, Xue F, Fan X, Gong Y
Cellular signalling 2022 Oct;98:110418

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Targeting APLN/APJ restores blood-testis barrier and improves spermatogenesis in murine and human diabetic models.

Song K, Yang X, An G, Xia X, Zhao J, Xu X, Wan C, Liu T, Zheng Y, Ren S, Wang M, Chang G, Cronin SJF, Penninger JM, Jing T, Ou X, Rao S, Liu Z, Zhao XY
Nature communications 2022 Nov 28;13(1):7335

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Neuropeptide apelin presented in the dopaminergic neurons modulates the neuronal excitability in the substantia nigra pars compacta.

Liu C, Kang NW, Wang Y, Xue Y, Chen XY, Chen L
Neuropharmacology 2022 Nov 15;219:109235

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TNF-α Suppresses Apelin Receptor Expression in Mouse Quadriceps Femoris-Derived Cells.

Koyama T, Uchida K, Itakura M, Miyagi M, Tazawa R, Inoue G, Fukushima K, Ohashi Y, Tsukada A, Takaso M
Current issues in molecular biology 2022 Jul 8;44(7):3146-3155

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Maternal exercise intergenerationally drives muscle-based thermogenesis via activation of apelin-AMPK signaling.

Son JS, Chae SA, Zhao L, Wang H, de Avila JM, Zhu MJ, Jiang Z, Du M
EBioMedicine 2022 Feb;76:103842

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Deubiquitylase OTUD3 prevents Parkinson's disease through stabilizing iron regulatory protein 2.

Jia F, Li H, Jiao Q, Li C, Fu L, Cui C, Jiang H, Zhang L
Cell death & disease 2022 Apr 30;13(4):418

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Apelin/APJ-Manipulated CaMKK/AMPK/GSK3β Signaling Works as an Endogenous Counterinjury Mechanism in Promoting the Vitality of Random-Pattern Skin Flaps.

Lou ZL, Zhang CX, Li JF, Chen RH, Wu WJ, Hu XF, Shi HC, Gao WY, Zhao QF
Oxidative medicine and cellular longevity 2021;2021:8836058

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Hsa_circ_0123190 acts as a competitive endogenous RNA to regulate APLNR expression by sponging hsa-miR-483-3p in lupus nephritis.

Zhang C, Gao C, Di X, Cui S, Liang W, Sun W, Yao M, Liu S, Zheng Z
Arthritis research & therapy 2021 Jan 13;23(1):24

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Apelin-13 induces mitophagy in bone marrow mesenchymal stem cells to suppress intracellular oxidative stress and ameliorate osteoporosis by activation of AMPK signaling pathway.

Chen L, Shi X, Xie J, Weng SJ, Xie ZJ, Tang JH, Yan DY, Wang BZ, Fang KH, Hong CX, Wu ZY, Yang L
Free radical biology & medicine 2021 Feb 1;163:356-368

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Rip 1-dependent endothelial necroptosis participates in ischemia-reperfusion injury of mouse flap.

Geng L, Zhang G, Yao M, Fang Y
Journal of dermatological science 2020 Jan;97(1):30-40

---

ELABELA ameliorates hypoxic/ischemic-induced bone mesenchymal stem cell apoptosis via alleviation of mitochondrial dysfunction and activation of PI3K/AKT and ERK1/2 pathways.

Fu J, Chen X, Liu X, Xu D, Yang H, Zeng C, Long H, Zhou C, Wu H, Zheng G, Wu H, Wang W, Wang T
Stem cell research & therapy 2020 Dec 14;11(1):541

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Maternal Inactivity Programs Skeletal Muscle Dysfunction in Offspring Mice by Attenuating Apelin Signaling and Mitochondrial Biogenesis.

Son JS, Chae SA, Wang H, Chen Y, Bravo Iniguez A, de Avila JM, Jiang Z, Zhu MJ, Du M
Cell reports 2020 Dec 1;33(9):108461

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Single-Cell RNA Sequencing Maps Endothelial Metabolic Plasticity in Pathological Angiogenesis.

Rohlenova K, Goveia J, García-Caballero M, Subramanian A, Kalucka J, Treps L, Falkenberg KD, de Rooij LPMH, Zheng Y, Lin L, Sokol L, Teuwen LA, Geldhof V, Taverna F, Pircher A, Conradi LC, Khan S, Stegen S, Panovska D, De Smet F, Staal FJT, Mclaughlin RJ, Vinckier S, Van Bergen T, Ectors N, De Haes P, Wang J, Bolund L, Schoonjans L, Karakach TK, Yang H, Carmeliet G, Liu Y, Thienpont B, Dewerchin M, Eelen G, Li X, Luo Y, Carmeliet P
Cell metabolism 2020 Apr 7;31(4):862-877.e14

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Apelin-13 Suppresses Neuroinflammation Against Cognitive Deficit in a Streptozotocin-Induced Rat Model of Alzheimer's Disease Through Activation of BDNF-TrkB Signaling Pathway.

Luo H, Xiang Y, Qu X, Liu H, Liu C, Li G, Han L, Qin X
Frontiers in pharmacology 2019;10:395

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APLNR is involved in ATRA-induced growth inhibition of nasopharyngeal carcinoma and may suppress EMT through PI3K-Akt-mTOR signaling.

Liu Y, Liu Q, Chen S, Liu Y, Huang Y, Chen P, Li X, Gao G, Xu K, Fan S, Zeng Z, Xiong W, Tan M, Li G, Zhang W
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Nov;33(11):11959-11972

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Molecular determinants of nephron vascular specialization in the kidney.

Barry DM, McMillan EA, Kunar B, Lis R, Zhang T, Lu T, Daniel E, Yokoyama M, Gomez-Salinero JM, Sureshbabu A, Cleaver O, Di Lorenzo A, Choi ME, Xiang J, Redmond D, Rabbany SY, Muthukumar T, Rafii S
Nature communications 2019 Dec 13;10(1):5705

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Apelin-13 Upregulates BDNF Against Chronic Stress-induced Depression-like Phenotypes by Ameliorating HPA Axis and Hippocampal Glucocorticoid Receptor Dysfunctions.

Dai TT, Wang B, Xiao ZY, You Y, Tian SW
Neuroscience 2018 Oct 15;390:151-159

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The Hippocampus is a Critical Site Mediating Antidepressant-like Activity of Apelin-13 in Rats.

Xiao ZY, Wang B, Fu W, Jin X, You Y, Tian SW, Kuang X
Neuroscience 2018 Apr 1;375:1-9

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Apelin attenuates the osteoblastic differentiation of aortic valve interstitial cells via the ERK and PI3-K/Akt pathways.

Yuan ZS, Zhou YZ, Liao XB, Luo JW, Shen KJ, Hu YR, Gu L, Li JM, Tan CM, Chen HM, Zhou XM
Amino acids 2015 Dec;47(12):2475-82

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20341-1-AP( APLNR Antibody) at dilution of 1:600

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The APLNR antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human APLNR. This antibody recognizes human, mouse antigen. The APLNR antibody has been validated for the following applications: ELISA, WB, IHC analysis.

Appendix

20341-1-AP.pdf

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