Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- BS2584 - Provider product page
- Provider
- Bioworld Technology, Inc
- Proper citation
- Bioworld Technology Cat#BS2584, RRID:AB_1663796
- Product name
- APLNR (C181) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide, corresponding to amino acids 150-200 of Human APLNR.
- Description
- The apelin receptor (APLNR) is a G protein-coupled integral membrane protein exhibiting a hypothalamic distribution in brain, glial cells, astrocytes and neuronal subpopulations. APLNR is bound by its cognate neuropeptide ligand, apelin, promoting receptor internalization to the nucleus and dose-dependent inhibition of forskolin-induced cAMP production. However, deletion studies of the apelin agonist have shown that internalization is not mandatory for decreasing vasopressin release, a hypotensive action of APLNR signaling. Further evidence for functional dissociation of APLNR stimulation and internalization was exhibited in vitro using mutational studies of a nuclear localization signal sequence. These findings may suggest the presence of multiple, functionally-differing conformational states for the receptor. Stress studies in rodents have shown APLNR is under negative regulation by glucocorticoids and may be involved in controlling hypothalamic function. APLNR also functions as an alternate coreceptor with CD4 for HIV-1 infection.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
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- Experimental details
- Western blot (WB) analysis of APLNR (C181) pAb at 1:500 dilutionLane1:A549 cell lysateLane2:Mouse lung tissue lysateLane3:Rat liver tissue lysate