711101

antibody from Invitrogen Antibodies

Targeting: APLNR AGTRL1, APJ, APJR, FLJ90771

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

711101

Provider

Invitrogen Antibodies

Product name

Apelin Receptor Recombinant Polyclonal Antibody (5HCLC)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

5HCLC

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Obesity and triple-negative-breast-cancer: Is apelin a new key target?

Gourgue F, Mignion L, Van Hul M, Dehaen N, Bastien E, Payen V, Leroy B, Joudiou N, Vertommen D, Bouzin C, Delzenne N, Gallez B, Feron O, Jordan BF, Cani PD
Journal of cellular and molecular medicine 2020 Sep;24(17):10233-10244

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on membrane enriched extracts (30 µg of lysate) of HepG2 (Lane 1), NTERA-2 (Lane 2), HEL.92.1.7 (Lane 3), K562 (Lane 4), HeLa (Lane 8) and tissue extracts of Mouse Liver (Lane 5), Mouse Testis (Lane 6) Rat Testis (Lane 7) and Mouse Lung (Lane 9). The blots were probed with Anti-APLNR Recombinant Rabbit Polyclonal Antibody (Product # 711101, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 43 kDa band corresponding to APLNR protein was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIGURE 2 Infusion of obesity-related levels of apelin favours TNBC growth in lean mice. A, Experimental design of PBS or apelin infusion (0.1 umol/kg/d) in lean balb/cJRj mice. B, Bodyweight gain during apelin infusion. C, 4T1 TNBC growth in Balb/cJRj mice. D, Expression of Aplnr protein in 4T1 & E0771 TNBC cells with U87MG cells as positive control. E, mRNA expression of Apln and Mki67 in 4T1 TNBC cells in vitro in response to 24 h of apelin treatment. F, BrdU incorporation assay in 4T1 TNBC cells in vitro in response to 24 h of apelin treatment. G, mRNA expression of Apln, tumoral progression markers (Mki67, Ptk2b, Pdgfra, Pdgfrb, Yap, Taz), apoptosis markers (Bax, Bcl2l1), lipid metabolism markers (Ldlr, Fasn, Acaca), glucose metabolism marker (Slc2a1) and immune markers (Ccl2 & Itgax) in 4T1 TNBC tumours. H, I, Tyrosine phosphorylation of 196 peptides from 4T1 TNBC tumours. J, Expression of Angpt1 and Pecam1 in 4T1 tumours. K, L, Immunohistochemistry of Pecam1 on 4T1 TNBC tumours. Data are presented as mean +- SEM. Number of mice per group for (B, C): Control: 15, Apelin: 16, for (E, F): n = 3, for (G-L) Control: 5-6, Apelin 5-6. Data were analysed using Student's t test for (B, G, J, K) or using one-way ANOVA for (E, F). Data were analysed using two-way ANOVA followed by Bonferroni post hoc test for (C). * P < 0.05; ** P < 0.01; *** P < 0.001