PA5-80343

antibody from Invitrogen Antibodies

Targeting: APLNR AGTRL1, APJ, APJR, FLJ90771

Immunoprecipitation

Antibody data

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Validation data

Product number: PA5-80343 - Provider product page
Provider: Invitrogen Antibodies
Product name: Apelin Receptor Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). This antibody has specificity for Human APJ Receptor/APLNR.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

APLNR protein structure - PA5-80343 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of Apelin receptor was achieved by transfecting K-562 with Apelin receptor specific siRNAs (Silencer® select Product # s1186 and s1188). Western blot analysis (Fig. a) was performed using Whole cell extracts from the Apelin receptor knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with Apelin Receptor Polyclonal Antibody (Product # PA5-80343, 0.2 µg/ml) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Apelin receptor.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-Apelin Receptor Polyclonal Antibody (Product # PA5-80343) and a 42 kDa band corresponding to Apelin receptor was observed across cell lines tested. Whole cell extracts (20 µg lysate) of Hep G2 (Lane 1), K-562 (Lane 2), PC-3 (Lane 3), Daudi (Lane 4) and Jurkat (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Jurkat expresses APLNR protein at lower levels as compared to Daudi. Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.2 µg/ml) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).