- PA5-114830 - Invitrogen Antibodies APLNR antibody

Gao Z, Zhong X, Tan YX, Liu D
International journal of molecular medicine 2021 Sep;48(3)

No comments: Submit comment

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry analysis of Apelin receptor in 293 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114830) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with Alexa Fluor 594 conjugated goat anti-rabbit IgG using a dilution of 1:600.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry analysis of Apelin receptor in 293 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114830) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with Alexa Fluor 594 conjugated goat anti-rabbit IgG using a dilution of 1:600.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Apelin receptor in mouse testis tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-114830) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Apelin receptor in rat testis tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-114830) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 Apelin-13 activates the expression of APJ. (A) The expression of APJ in the kidney tissues was detected with the immunohistochemical assays (magnification, x200). (B) The production of NO was detected with the commercial kits. (C) Levels of Ang II and ET-1 were determined with commercial kits. ** P

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 Application of apelin-13 promotes the expression of eNOS. (A) The expression of AT1R and eNOS was detected with the western blotting. (B) The levels of APJ, TGBR, E-cadherin and alpha-SMA in kidney tissues of rats were detected with western blotting. * P

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5 Apelin-13 alleviates the higher glucose-induced injury of endothelial cells of glomerular vessels. (A) Cell viability was detected with the Cell Counting Kit-8 assay. (B) hRGECs were detected with the lumen formation experiments (magnification, x4). (C) The expression of AT1R, eNOS, APJ, TGBR, E-cadherin and alpha-SMA in these cells was determined with the western blotting. * P

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 6 Apelin-13 relieves the injury of endothelial cells of glomerular vessels by suppressing fibrosis. (A) Cell viability of endothelial cells of glomerular vessels was determined with Cell Counting Kit-8 assay. (B) hRGECs were detected with the lumen formation experiments (magnification, x4). (C) The levels of AT1R, eNOS, APJ, TGBR, E-cadherin and alpha-SMA in these cells were determined with western blotting. * P

PA5-114830