PA5-31589

antibody from Invitrogen Antibodies

Targeting: SEC23B CDA-II, CDAII, CDAN2, HEMPAS

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-31589

Provider

Invitrogen Antibodies

Product name

SEC23B Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: Raji. Predicted reactivity: Mouse (96%), Rat (97%), Zebrafish (86%), Xenopus laevis (92%), Pig (98%), Chicken (93%), Chimpanzee (99%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SEC23B using 30 µg of Raji lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a SEC23B polyclonal antibody (Product # PA5-31589) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using SEC23B Polyclonal Antibody (Product # PA5-31589). Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SEC23B Polyclonal Antibody (Product # PA5-31589) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SEC23B antibody detects SEC23B protein at cytoplasm on Ca922 oral cancer xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Ca922 oral cancer xenograft. SEC23B antibody (Product # PA5-31589) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.