Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-31589 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SEC23B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Raji.
- Concentration
- 1 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SEC23B using 30 µg of Raji lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a SEC23B polyclonal antibody (Product # PA5-31589) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SEC23B Polyclonal Antibody (Product # PA5-31589). Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SEC23B Polyclonal Antibody (Product # PA5-31589) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SEC23B antibody detects SEC23B protein at cytoplasm on Ca922 oral cancer xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Ca922 oral cancer xenograft. SEC23B antibody (Product # PA5-31589) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
