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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NB100-75623 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-75623, RRID:AB_1049234
- Product name
- Chicken Polyclonal NKCC1/SLC12A2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Chicken Polyclonal to NKCC1
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Simian
- Host
- Chicken/Avian
- Vial size
- 0.05 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NKCC1/SLC12A2 Antibody [NB100-75623] - E. coli-derived fusion protein as test antigen. Affinity-purified IgY dilution 1:2000, Goat anti-IgY-HRP dilution 1:1000. Colorimetric method for signal development (anti-SLC12A2).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: NKCC1/SLC12A2 Antibody [NB100-75623] - Human colon tissue was incubated with anti-NKCC1 antibody at 5 ug/mL (1:200) overnight at 4C and detected with Alexa Fluor 488 Goat anti-Chicken (1:200 Invitrogen, cat# A11039). Nuclei stained with DAPI. Image from verified customer review.
