Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
Submit
Validation data
Reference
Comment
Report error
- Product number
- R31290 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- NKCC1 Antibody / SLC12A2
- Antibody type
- Polyclonal
- Description
- This highly specific NKCC1 antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the NKCC1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of NKCC1 antibody and HeLa cell lysate. Expected/observed size ~130KD
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of NKCC1 antibody and human A549 cell lysate. Predicted molecular weight ~131 kDa but may be observed at higher molecular weights due to glycosylation.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human colon cancer tissue with NKCC1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: NKCC1 antibody testing of human intestinal cancer tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: NKCC1 antibody testing of rat intestine tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-F testing of NKCC1 antibody and rat intestine tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human colorectal adenocarcinoma tissue with NKCC1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE mouse colon tissue with NKCC1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE rat colon tissue with NKCC1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
