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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- 14743-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DRG2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MGILEKISE IEKEIARTQK NKATEYHLGL LKAKLAKYRA QLLEPSKSAS SKGEGFDVMK SGDARVALIG FPSVGKSTFL SLMTSTASEA ASYEFTTLTC IPGVIEYKGA NIQLLDLPGI IEGAAQGKGR GRQVIAVART ADVIIMMLDA TKGEVQRSLL EKELESVGIR LNKHKPNIYF KPKKGGGISF NSTVMLTQCS EKLVQLILHE YKIFNAEVLF REDCSPDEFI DVIVGNRVYM PCLYVYNKID QISMEEVDRL ARKPNSVVIS CGMKLNLDYL LEMLWEYLAL TCIYTKKRGQ RPDFTDAIIL RKGASVEHVC HRIHRSLASQ FKYALVWGTS TKYSPQRVGL THTMEHEDVI QIVKK (1-364 aa encoded by BC000493)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.24 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 14743-1-AP (DRG2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 14743-1-AP (DRG2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol) fixed HepG2 cells using 14743-1-AP (DRG2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 14743-1-AP ( DRG2 antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 14743-1-AP ( DRG2 antibody) at dilution of 1:200 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-DRG2 (IP:14743-1-AP, 4ug; Detection:14743-1-AP 1:2000) with mouse brain tissue lysate 2640ug.
