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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [4]
- Other assay [1]
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- Product number
- PA5-52478 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ABCA3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MLRLTLGEYG RTVVPFSVPG TSQLGQQLSE HLKDALQAEG QEPREVLGDL EEFLIFRASV EGGGFNERCL VAASFRDVGE RTVVNALFNN QAYHSPATAL AVVDNLLFKL LCGPHASIVV SNFPQPRSAL QAAKDQFNEG Highest antigen sequence identity to the following orthologs: Mouse - 82%, Rat - 85%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Co-culture of type I and type II pneumocytes as a model of alveolar epithelium.
Brookes O, Boland S, Lai Kuen R, Miremont D, Movassat J, Baeza-Squiban A
PloS one 2021;16(9):e0248798
PloS one 2021;16(9):e0248798
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of ABCA3 in human cell line U-2 OS shows positivity in cytoplasm & nucleus but excluded from the nucleoli. Samples were probed using an ABCA3 Polyclonal Antibody (Product # PA5-52478).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of ABCA3 in human cell line HUVEC TERT2 using an ABCA3 Polyclonal Antibody (Product # PA5-52478) shows localization to nucleoplasm and cytosol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ABCA3 in human lung and liver tissues using ABCA3 Polyclonal Antibody (Product # PA5-52478). Corresponding ABCA3 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ABCA3 in human lung using ABCA3 Polyclonal Antibody (Product # PA5-52478) shows strong positivity in pneumocytes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ABCA3 in human cerebellum using ABCA3 Polyclonal Antibody (Product # PA5-52478) shows positivity in Purkinje cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ABCA3 in human liver using ABCA3 Polyclonal Antibody (Product # PA5-52478) shows weak cytoplasmic positivity in hepatocytes.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 10.1371/journal.pone.0248798.g006 Fig 6 NCI-H441 cells and co-cultures produce surfactant. Immunofluorescent labelling of the lamellar body component ABCA3 (red) in normal human tissue samples (a) and in NCI-H441 ALI cultures (b). HTII-280, another type-II specific antigen (white) is present in the apical surface of type-II cells in normal human tissue (a) and in NCI-H441 (c) and cocultures after 14d (d). Immuno-labelling of pro-SPC reveals production of surfactant protein in cocultures (e) and normal human lung tissue (f). Electron microscopy reveals the presence of lamellar bodies beneath the surface of co-cultures of hAELVi and NCI-H441 cells grown in ALI conditions (g). RTqPCR reveals expression of both surfactant protein C and ABCA3 (h, n = 3 replicates within 3 independent experiments), and western blotting with an anti-ABCA3 polyclonal antibody reveals post-translational processing of ABCA3 (i).
