PA5-70914

antibody from Invitrogen Antibodies

Targeting: P2RY10 P2Y10

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-70914

Provider

Invitrogen Antibodies

Product name

P2Y10 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This target displays homology in the following species: Human: 100%; Mouse: 86%; Rat: 100%

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

G-protein-coupled receptor P2Y10 facilitates chemokine-induced CD4 T cell migration through autocrine/paracrine mediators.

Gurusamy M, Tischner D, Shao J, Klatt S, Zukunft S, Bonnavion R, Günther S, Siebenbrodt K, Kestner RI, Kuhlmann T, Fleming I, Offermanns S, Wettschureck N
Nature communications 2021 Nov 23;12(1):6798

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of P2Y10 on HepG2 cells. The sample was probed with a P2Y10 polyclonal antibody (Product # PA5-70914) using a primary antibody dilution of 1.0 µg/mL.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 1 Generation and basal characterization of CD4-P2Y10-KOs. a Expression of selected GPCRs for chemokines or lipids and metabolites in naive and spinal cord-infiltrating CD4 T cells was determined by Nanostring mRNA quantification (naive CD4: splenic CD4 T cells from two healthy mice; sc-inf. CD4: spinal cord-infiltrating CD4 T cells pooled from four individual mice at EAE day 14). b Design of the floxed P2Y10 allele. c , d Reduction of P2Y10 expression in CD4 T cells from control and CD4-P2Y10-KO mice was judged by immunoblotting ( c , GAPDH as loading control) and RNA sequencing in isolated splenic CD4 T cells ( d , n = 3; ** p = 0.0021). Total leukocyte numbers ( e ) and proportion of lymphocyte populations in thymus ( f ), inguinal lymph nodes ( g ), or spleen ( h ) ( n = 6-12). Cytokine production ( i , n = 5-6) and proliferation ( j , n = 7) in CD3/CD28-activated CD4 T cells. Percentage of IFNgamma/ IL-17A/FoxP3-expressing cells ( k , n = 7-9) and proliferating cells ( l , n = 4-5) after Th1 and Th17 differentiation, respectively. Data are means +- SEM (in ( a ) only means); comparisons between genotypes were performed using two-tailed unpaired Student's t-test. n , number of mice per group.