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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB10158-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human P2Y10/P2RY10 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human P2RY10 in Flow Cytometry and Immunohistochemistry.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
O00398- Isotype
- IgG
- Antibody clone number
- 639329
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- P2Y10/P2RY10 in Human Thymus. P2Y10/P2RY10 was detected in immersion fixed paraffin-embedded sections of human thymus using Mouse Anti-Human P2Y10/P2RY10 Monoclonal Antibody (Catalog # MAB10158) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of P2Y10/P2RY10 in HEK293 Human Cell Line Transfected with Human P2Y10/P2RY10 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with P2Y10/P2RY10 and eGFP was stained with (A) Mouse Anti-Human P2Y10/P2RY10 Monoclonal Antibody (Catalog # MAB10158) or (B) Mouse IgG2B Isotype Control Antibody (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
