A302-922A

antibody from Invitrogen Antibodies

Targeting: RABGEF1 rabex-5, RABEX5

Western blot (Recommended by provider)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A302-922A

Provider

Invitrogen Antibodies

Product name

RABGEF1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human RABGEF1 by immunohistochemistry. Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti- RABGEF1 (Cat. No. A302-922A Lot2) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human RABGEF1 by western blot of immunoprecipitates. Samples:Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RABGEF1 antibody A302-922A (lot A302-922A-2) used for IP at 3 µg per reaction. RABGEF1 was also immunoprecipitated by rabbit anti-RABGEF1 antibody A302-921A. For blotting immunoprecipitated RABGEF1, A302-921A was used at 1 µg/ml. Detection:Chemiluminescence with an exposure time of 3 minutes.